期刊 GRAFTING OF HYDROPHILIC MONOMERS ONTO POLYURETHANE MEMBRANES BY SOLUTION OR PRE-ABSORBING METHODS FOR ACCELERATION OF CELL COMPATIBILITY  被引量:1

GRAFTING OF HYDROPHILIC MONOMERS ONTO POLYURETHANE MEMBRANES BY SOLUTION OR PRE-ABSORBING METHODS FOR ACCELERATION OF CELL COMPATIBILITY

作  者:Chang-you Gao Jian-jun Guan Jia-cong Shen Department of Polymer Science and Engineering Zhejiang University Hangzhou 310027, China 

出  处:《高分子科学:英文版》2001年第5期493-498,共6页

GRAFTING OF HYDROPHILIC MONOMERS ONTO POLYURETHANE MEMBRANES BY SOLUTION OR PRE-ABSORBING METHODS FOR ACCELERATION OF CELL COMPATIBILITY

基  金:This project was supported by the National Natural Science Foundation of China (No. 59803006), the Major State Basic Research Program of China (G1999054305) and the Natural Science Foundation of Zhejiang Province.

摘  要:Two grafting methods, i.e. solution grafting and pre-adsorbing, are introduced to covalently immobilize hydrophilic polymers on segmented polyurethane (SPU) to modify its hydrophilicity and to improve its cell compatibility. Solution grafting results in higher degree of grafting and rougher surface morphology. Cell culture evaluation demonstrates that the modified membranes thus obtained are disadvantageous to the endothelial cell (ECs) growth probably because the hydrophilic groups on the surface are over-crowded. However, pre-absorbing grafting generates lower degree of grafting, which is detected and confirmed by ATR-FTIR spectra and water contact angle. Scanning electron microscopic (SEM) measurement shows that the latter method produces a plane and smooth morphology, which is similar to the SPU control sample controlled. When grafting with lower monomer concentration, ECs could grow on SPU-g-PHEA [poly(2-hydroxyethyl acrylate)], SPU-g-PAAm (polyacrylamide), SPU-g-PDMA [poly(2-(dimethylamino)ethyl methacrylate)] or quaternized SPU-g-PDMA surface with elongated cell shapes. Hence, the cell compatibility of SPU is improved and a useful method to construct a cell compatible layer on the polymer surface has been developed.

Two grafting methods, i.e. solution grafting and pre-adsorbing, are introduced to covalently immobilize hydrophilic polymers on segmented polyurethane (SPU) to modify its hydrophilicity and to improve its cell compatibility. Solution grafting results in higher degree of grafting and rougher surface morphology. Cell culture evaluation demonstrates that the modified membranes thus obtained are disadvantageous to the endothelial cell (ECs) growth probably because the hydrophilic groups on the surface are over-crowded. However, pre-absorbing grafting generates lower degree of grafting, which is detected and confirmed by ATR-FTIR spectra and water contact angle. Scanning electron microscopic (SEM) measurement shows that the latter method produces a plane and smooth morphology, which is similar to the SPU control sample controlled. When grafting with lower monomer concentration, ECs could grow on SPU-g-PHEA [poly(2-hydroxyethyl acrylate)], SPU-g-PAAm (polyacrylamide), SPU-g-PDMA [poly(2-(dimethylamino)ethyl methacrylate)] or quaternized SPU-g-PDMA surface with elongated cell shapes. Hence, the cell compatibility of SPU is improved and a useful method to construct a cell compatible layer on the polymer surface has been developed.

关 键 词:SEGMENTED polyurethane  CELL compatibility  Surface grafting  ENDOTHELIAL CELL 

segmented polyurethane  cell compatibility  surface grafting  endothelial cell 

分 类 号:O63[理学—高分子化学]

 

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