期刊 TIMP-4在妊娠及产后小鼠卵巢中表达的动态变化  被引量:1

CHANGING TRENDS OF THE EXPRESSION OF TIMP-4 IN MOUSE OVARY DURING PREGNANT AND POSTPARTUM PERIOD

作  者:卜淑敏[1] 胡增[2] 张凡[1] 段恩奎[2] 

机构地区:[1]首都体育学院重点实验室,北京100088 [2]中国科学院动物研究所计划生育生殖生物学国家重点实验室,北京100080

出  处:《中国应用生理学杂志》2008年第2期248-252,共5页Chinese Journal of Applied Physiology

CHANGING TRENDS OF THE EXPRESSION OF TIMP-4 IN MOUSE OVARY DURING PREGNANT AND POSTPARTUM PERIOD

基  金:中国第35批博士后基金(2004035099);首都体育学院人才引进项目资助

摘  要:目的:研究金属蛋白酶组织抑制剂-4(TIMP-4)在妊娠和产后小鼠卵巢中表达的变化规律,探讨其在黄体中的作用。方法:采用逆转录-聚合酶链反应(RT-PCR)和间接免疫荧光染色两种方法,分别检测了TIMP-4mRNA和蛋白在妊娠和产后小鼠卵巢中的时空表达。结果:RT-PCR显示,妊娠各个时期以及产后第1 d小鼠卵巢中均有TIMP-4mRNA的表达,其中第8 d表达水平最高,第14 d开始下降,产后第1 d降至最低水平。间接免疫荧光染色进一步显示,TIMP-4蛋白定位于妊娠各时期和产后第1 d卵巢的膜细胞、黄体、基质以及黄体化的卵泡中,但在颗粒细胞中不表达。此外,在妊娠黄体中,TIMP-4蛋白与TIMP-4mRNA的变化趋势基本一致。结论:TIMP-4在妊娠小鼠卵巢中的表达具有时空特异性,其可能参与妊娠小鼠黄体的形成和功能维持。

Aim: The changes of tissue inhibitor of metalloproteinase-4 (TIMP-4) expression in mouse ovary during pregnant and postpartum period were studied to investigate the role of TIMP-4 in corpus luteum (CL). Methods: RT-PCR was used to deter mine the change of TIMP-4 rnRNA and indirect immunofluoresence was used to observe the change of TIMP-4 protein. The expression of TIMP-4 rnRNA was observed in various periods throughout the stage of pregnancy and postpartum day 1. Results: The expression of TIMP-4 was gradually enhanced from day 1 to day 8, reached a maximal expression at day 8, while decreased at day 11 and to the lowest level at postpartum day 1. Indirect immunofluorescence results further indicated that TIMP-4 protein was localized to CL and theca-intera cells in various periods throughout the pregnancy and postpartum day 1. In addition, the change pattern of TIMP-4 protein agreed with that of the TIMP-4 mRNA in pregnancy CL. Conclusion: The expression of TIMP-4 in mouse ovary during pregnancy and postpartum is in spatio-temporal pattern and it may be involved in the formation and function maintain of CL during pregnancy in mice.

关 键 词:妊娠 小鼠 卵巢 金属蛋白酶组织抑制剂-4 

pregnancy  mouse  ovary  TIMP-4 

分 类 号:R339.2[医药卫生—人体生理学]

 

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