期刊 骨髓间充质干细胞分化为血管内皮细胞的实验研究  被引量:129

An experimental study on the differentiation of bone marrow mesenchymal stem cells into vascular endothelial cells

作  者:方利君[1] 付小兵[2] 孙同柱[2] 李建福[2] 程飚[2] 杨银辉[2] 王玉新[3] 

FANG Li-jun,FU XIAO-bing,SUN TONG-zhu,LI Jian-fu,CHENG Biao,YANG Yin-hui,WANG Yu-xin.Department of Stomatology,The Second Affiliated Clinical College,Chinese Medical University,Shen Yang 110003,Liao Ning Province;Burns Institute, 304th Hospital,Beijing 100037 P.R.China

机构地区:[1]110003,沈阳,中国医科大学附属第二临床学院口腔科 [2]解放军第三○四医院 [3]中国医科大学附属第一临床学院口腔颌面外科

出  处:《中华烧伤杂志》2003年第1期 22-24,共3页Chinese Journal of Burns

An experimental study on the differentiation of bone marrow mesenchymal stem cells into vascular endothelial cells

基  金:国家重点基础研究发展计划(973计划),国家自然科学基金,国家自然科学基金

摘  要:目的探讨骨髓间充质干细胞(MSCs)分化为肉芽组织中血管内皮细胞的可能性,及其参与创面修复的可能机制.方法抽取小型香猪的骨髓,经密度梯度离心分离、纯化MSCs,体外培养扩增后,应用溴脱氧尿嘧啶(BrdU)标记技术标记细胞.于已抽取骨髓的小型香猪背部制作全层皮肤缺损创面,即刻以纤维蛋白胶为载体,将已标记的MSC回植到供体动物创面上.术后2、4、6、8、12用切取创面组织,行BrdU和因子Ⅷ(FⅧ)免疫组织化学染色,进行对比观察. 结果BrdU阳性的MSCs多聚集在创面肉芽组织中的小血管周围,日有个别血管内皮细胞也呈现BrdU阳性.部分BrdU阳性的MSCs胞浆中亦有FⅧ表达. 结论创面愈合过程中,MSCs与肉芽组织中小血管的形成密切相关.在创面微环境下,MSCs可分化为血管内皮细胞,并参与创面修复.

Objective To investigate the feasibility of differentiation of bone marrow mesenchymal stem cells (MSCs) into vascular endothelial cells and the mechanism of its involvement in wound healing. Methods Porcine MSCs were harvested from porcine marrow, and they were isolated and purified by density gradient centrifugation. After being cultured and amplified in vitro, the MSCs were labelled with BrdU (5-bromodeoxy-uridine). Full skin loss wound was created on the back of the mini-swine whose bone marrow was obtained. The labelled MSCs with fibrin glue as the vector were regrafted back to the donor animal wound. The wound tissue specimens were harvested at 2, 4, 6, 8 and 12 post-operation weeks and were immunohistochemically stained by BrdU and factor Ⅷ (FⅧ) for comparative study. Results Most BrdU positive cells aggregated around small blood vessels in the granulation tissue of the wounds. Only individual vascular endothelial cells were BrdU positive. There was FⅧ expression in the cytoplasm of BrdU positive cells . Conclusion MSCs were closely correlated with the formation of small blood vessels in granulation tissue during wound healing process. The porcine MSCs possessed the potential to differentiate into vascular entoehelial cells and to participate in wound healing under the micro-enviroment of the wound. [

关 键 词:骨髓间充质干细胞 分化 血管内皮细胞 实验研究 分离 培养 创面 修复 

Stem Cells  Bone Marrow  Vascular endothelial cells  Wound repair 

分 类 号:Q813.11[生物学—生物工程] R641[医药卫生—临床医学;医药卫生—外科学]

 

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